Authors
1
Nutrition and food science Dept.- faculty of home economics- menoufia university
2
nutrition and food science department, faculty of home economics, menoufia university
3
nutrition department, Menoufia University Hospitals
Abstract
Processing/cooking starchy foods at high temperatures such as French fries and other baked goods produces toxic chemicals, including acrylamide. Therefore, the present study aimed to investigate the potential protective effects of Eruca sativa leaves ethanolic extract (ESLE), against acrylamide-induced biochemical and histological alterations in rat testis. Rats (n=30), were randomly assigned to five equal groups as follow: Group 1, served as normal control; Group 2, model control, received 50 mg/kg BW/day of ACR to induce infertility; groups 3 to 5 received ESLE-1, ESLE-2 and clomid (Clo, infertility drug reference) at concentrations of 250, 500 and 1.2 mg/kg BW/day by oral gavage for 28 days, respectively followed by ACR. Treatment of rats with ACR caused a significant increased (p≤0.05) in sperm abnormalities and testis malonaldehyde (MDA) by 471.32 and 292.1%, respectively compared to the normal animals. Also, sperm count, sperm motility, serum testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) content were significant (p≤0.05) decreased by -93.78, -68.95, -80.84, -76.98 and -89.16%, respectively. Furthermore, testis antioxidant enzymes (Superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) activities were decreased by -79.29, -81.09 and -80.74%, respectively compared to the normal control rats. Additionally, liver dysfunction and harmful histopathological changes in testicular tissue were observed as ACR treatment. However, intervention with ESLE (500 mg/kg) significantly (p≤0.05) restored most of these parameters to levels close to normal. In conclusion, these observations serve as a foundation for using ESLE to treat and/or prevent ACR toxicity.
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Main Subjects
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